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Spectrophotometer Fluorescence F-2710

Spectrophotometer Fluorescence F-2710

Spectrophotometer Fluorescence F-2710

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Description

Spesifikasi Teknis Spectrophotometer Fluoresensi F-2710

1. Sistem Optik

  • Tipe: Single-beam dengan desain right-angle detection (90°).

  • Sumber Cahaya:

    • Lampu Xenon (Xe): 150 W, pulsed untuk stabilitas tinggi dan umur panjang.

    • Rentang Eksitasi: 200–750 nm.

  • Monokromator:

    • Eksitasi & Emisi: Grating blazed dengan focal length 200 mm.

    • Bandwidth: 5 nm, 10 nm, 20 nm (adjustable untuk eksitasi dan emisi).

  • Detektor:

    • Photomultiplier Tube (PMT): Rentang deteksi 220–730 nm.

    • Sensitivitas: High-gain PMT dengan reduksi noise hingga 10,000:1 (signal-to-noise ratio).

2. Performa Pengukuran

  • Rentang Pengukuran:

    • Intensitas Fluoresensi: 0–3,000,000 counts (linier hingga 1,000,000 counts).

    • Rentang Dinamis: >10⁵.

  • Akurasi Panjang Gelombang:

    • Eksitasi/Emisi: ±2 nm.

    • Reproduksibilitas: ±0.5 nm.

  • Kecepatan Scan: 1,000 nm/menit (maksimal).

3. Fitur Operasional

  • Modus Pengukuran:

    • Fluorescence Spectrum (scan eksitasi/emisi).

    • Time Course (kinetika fluoresensi).

    • Multi-Wavelength (pengukuran simultan pada beberapa λ).

  • Koreksi Spektrum: Opsional koreksi spektrum emisi untuk respons detektor.

  • Suhu Sampel: Kontrol suhu opsional (dengan aksesori sel Peltier).

4. Kompartemen Sampel

  • Holder Kuplet: Standar 10 mm (kuplet quartz atau plastik).

    • Volume Minimum: 50 µL (dengan adapter micro-volume).

  • Fitur Tambahan:

    • Stirrer magnetik opsional untuk pengadukan sampel.

    • Sistem aliran kontinu (flow cell) untuk analisis otomatis.

5. Kontrol & Perangkat Lunak

  • Antarmuka Pengguna:

    • Layar LCD dengan keypad untuk kontrol manual.

    • Konektivitas: RS-232/USB untuk koneksi ke PC.

  • Software:

    • Hitachi FL Solutions: Untuk akuisisi data, analisis spektrum, dan ekspor data.

    • Fitur: 3D scan (eksitasi-emisi matrix), kalibrasi otomatis.

6. Dimensi & Berat

  • Dimensi: 350 mm (W) × 450 mm (D) × 250 mm (H).

  • Berat: ~15 kg.

7. Persyaratan Daya

  • Listrik: 100–240 V AC, 50/60 Hz.

  • Daya: 300 W.

8. Lingkungan Operasi

  • Suhu: 15–35°C.

  • Kelembapan: 20–80% RH (non-kondensasi).

9. Aplikasi

  • Kimia Analitik: Kuantifikasi senyawa fluoresen (contoh: quinine, fluorescein).

  • Bioteknologi: Pengukuran DNA (dengan pewarna SYBR Green), protein (BCA assay).

  • Lingkungan: Deteksi polutan (contoh: PAH, logam berat).

  • Farmasi: Uji kemurnian obat.

10. Aksesori Standar/Opsional

  • Standar:

    • Kuplet quartz 10 mm, kabel data, perangkat lunak dasar.

  • Opsional:

    • Microplate reader adapter untuk 96-well plate.

    • Peltier temperature controller (±0.1°C akurasi).

    • Fiber optic probe untuk sampel padat atau in-situ.

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